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Inne
Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI-MS.
Autorzy
Rok wydania
2011
Czasopismo
Numer woluminu
17
Strony
445-453
DOI
10.1002/psc.1342
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
A series of model peptides in the form of quaternary ammonium salts at the N‐terminus was efficiently prepared by the solid‐phase synthesis. Tandem mass spectrometric analysis of the peptide quaternary ammonium derivatives was shown to provide sequence confirmation and enhanced detection. We designed the 2‐(1,4‐diazabicyclo[2.2.2] octylammonium)acetyl quaternary ammonium group which does not suffer from neutral losses during MS/MS experiments. The presented quaternization of 1,4‐diazabicyclo[2.2.2]octane (DABCO) by iodoacetylated peptides is relatively easy and compatible with standard solid‐phase peptide synthesis. This methodology offers a novel sensitive approach to analyze peptides and other compounds.
Słowa kluczowe
quaternary ammonium salts, Derivatization of peptides, peptide fragmentation, sequencing
Adres publiczny
https://doi.org/10.1002/psc.1342
Strona internetowa wydawcy
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