Repozytorium
Wyszukaj
Kolekcje
Inne
Hydrogen-deuterium exchange of α-carbon protons and fragmentation pathways in N-methylated glycine and alanine-containing peptides derivatized by quaternary ammonium salts.
Autorzy
Rok wydania
2014
Czasopismo
Numer woluminu
49
Strony
529-536
DOI
10.1002/jms.3371
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Recently, we developed a selective and efficient method of hydrogen‐deuterium exchange (HDX) at the α‐carbon (α‐C) of sarcosine residue (N‐methylglycine) in model peptides [Bąchor et al. J . Mass Spectrom . 2014, 49 , 43]. Here, we report the influence of quaternary ammonium (QA) group on HDX at the α‐C of sarcosine and N‐methylalanine in peptides. The obtained results suggest a significant acceleration of the HDX in sarcosine residue caused by the presence of QA. The effect depends on the distance between the sarcosine residue and QA moiety. The deuterons, introduced at α‐C, are resistant to the back‐exchange in acidic aqueous solution. The collision induced dissociation of the deuterium‐labeled analogs of QA‐tagged oligosarcosine peptides without mobile hydrogen revealed the mobilization of the hydrogens localized at α‐C of sarcosine residue.
Słowa kluczowe
HDX of peptides, quaternary ammonium salts, ESI‐MS/MS, sarcosine, charge-remote fragmentation
Adres publiczny
http://dx.doi.org/10.1002/jms.3371
Strona internetowa wydawcy
Podobne publikacje
Gas-phase fragmentation of oligoproline peptide ions lacking easily mobilizable protons.
Rudowska Magdalena, Wieczorek Robert, Kluczyk Alicja, Stefanowicz Piotr, Szewczuk Zbigniew
The unusual hydrogen-deuterium exchange of α-carbon protons inN-substituted glycine-containing peptides.
Bąchor Remigiusz, Setner Bartosz, Kluczyk Alicja, Stefanowicz Piotr, Szewczuk Zbigniew
Sensitive detection of charge derivatized peptides at the attomole level using nano-LC-ESI-MRM analysis.
Bąchor Remigiusz, Mielczarek Przemysław, Rudowska Magdalena, Silberring Jerzy, Szewczuk Zbigniew