Repozytorium
Wyszukaj
Kolekcje
Inne
The competition of charge remote and charge directed fragmentation mechanisms in quaternary ammonium salt derivatized peptides - an isotopic exchange study.
Autorzy
Rok wydania
2011
Czasopismo
Journal of the American Society for Mass Spectrometry
Numer woluminu
22
Strony
2103-2107
DOI
10.1007/s13361-011-0245-2.
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Derivatization of peptides as quaternary ammonium salts (QAS) is a promising method for sensitive detection by electrospray ionization tandem mass spectrometry (Cydzik et al. J. Pept. Sci. 2011, 17, 445-453). The peptides derivatized by QAS at their N-termini undergo fragmentation according to the two competing mechanisms - charge remote (ChR) and charge directed (ChD). The absence of mobile proton in the quaternary salt ion results in ChR dissociation of a peptide bond. However, Hofmann elimination of quaternary salt creates an ion with one mobile proton leading to the ChD fragmentation. The experiments on the quaternary ammonium salts with deuterated N-alkyl groups or amide NH bonds revealed that QAS derivatized peptides dissociate according to the mixed ChR-ChD mechanism. The isotopic labeling allows differentiation of fragments formed according to ChR and ChD mechanisms.
Słowa kluczowe
Quaternary ammonium salts, Derivatization of peptides, Peptide sequencing
Adres publiczny
http://dx.doi.org/10.1007/s13361-011-0245-2.
Strona internetowa wydawcy
Podobne publikacje
Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI-MS.
Cydzik Marzena, Rudowska Magdalena, Stefanowicz Piotr, Szewczuk Zbigniew
The 5-azoniaspiro[4.4]nonyl group for improved MS peptide analysis : a novel non-fragmenting ionization tag for mass spectrometric sensitive sequencing of peptides.
Setner Bartosz, Rudowska Magdalena, Kluczyk Alicja, Stefanowicz Piotr, Szewczuk Zbigniew
Peptides derivatized with bicyclic quaternary ammonium ionization tags. Sequencing via tandem mass spectrometry.
Setner Bartosz, Rudowska Magdalena, Klem Ewelina, Cebrat Marek, Szewczuk Zbigniew