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Inne
The hydrogen-deuterium exchange at α-carbon atom in N, N,N,-trialkylglycine residue: ESI-MS studies.
Autorzy
Rok wydania
2012
Czasopismo
Journal of the American Society for Mass Spectrometry
Numer woluminu
23
Strony
1024-1028
DOI
10.1007/s13361-012-0359-1
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Derivatization of peptides as quaternary ammonium salts (QAS) is a known method for sensitive detection by electrospray ionization tandem mass spectrometry. Hydrogens at α-carbon atom in N,N,N-trialkylglycine residue can be easily exchanged by deuterons. The exchange reaction is base-catalyzed and is dramatically slow at lower pH. Introduced deuterons are stable in acidic aqueous solution and are not back-exchanged during LC-MS analysis. Increased ionization efficiency, provided by the fixed positive charge on QAS group, as well as the deuterium labeling, enables the analysis of trace amounts of peptides.
Słowa kluczowe
Quaternary ammonium salts, HDX, Betaine, Charge-tagged peptides
Adres publiczny
http://dx.doi.org/10.1007/s13361-012-0359-1
Strona internetowa wydawcy
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