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Derivatization of peptides for improved detection by mass spectrometry.
Autorzy
Redaktorzy
Rok wydania
2016
Wydawca
Royal Society of Chemistry
Miejsce wydania
Cambridge
Strony
36-74
DOI
10.1039/9781782622680-00036
Tytuł książki
Amino acids, peptides and proteins. Vol. 40.
ISBN
978-1-78262-059-4
Kolekcja
Język
Angielski
Typ publikacji
Rozdział książki
Mass spectrometry is a sensitive and specific method of proteome analysis. Various strategies are applied to increase the ionization efficiency of peptides and determine or compare their concentrations in complex samples, involving modifications introducing fixed charge for signal intensification and isotopic labeling for quantitative analysis. Scientific concepts involved in design and optimization of peptide markers, including solid phase synthesis and isotopic exchange are discussed. From the challenges related to the analysis of post-translational modifications emerge the perspectives for the development and validation of efficient derivatization reagents.
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