Repozytorium
Wyszukaj
Kolekcje
Inne
Quaternary ammonium isobaric tag for a relative and absolute quantification of peptides.
Autorzy
Rok wydania
2018
Czasopismo
Numer woluminu
53
Strony
115-123
DOI
10.1002/jms.4040
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Isobaric labeling quantification of peptides has become a method of choice for mass spectrometry-based proteomics studies. However, despite of wide variety of commercially available isobaric tags, none of the currently available methods offers significant improvement of sensitivity of detection during MS experiment. Recently, many strategies were applied to increase the ionization efficiency of peptides involving chemical modifications introducing quaternary ammonium fixed charge. Here, we present a novel quaternary ammonium based isobaric tag for relative and absolute quantification of peptides (QAS-iTRAQ 2-plex). Upon collisional activation, the new stable benzylic-type cationic reporter ion is liberated from the tag. Deuterium atoms were used to offset the differential masses of an reporter group. We tested the applicability of QAS-iTRAQ 2-plex reagent on a series of model peptides as well as bovine serum albumin tryptic digest. Obtained results suggest usefulness of this isobaric ionization tag for relative and absolute quantification of peptides.
Słowa kluczowe
Derivatization of peptides, isobaric tags, Peptide sequencing
Adres publiczny
https://doi.org/10.1002/jms.4040
Strona internetowa wydawcy
Podobne publikacje
The 5-azoniaspiro[4.4]nonyl group for improved MS peptide analysis : a novel non-fragmenting ionization tag for mass spectrometric sensitive sequencing of peptides.
Setner Bartosz, Rudowska Magdalena, Kluczyk Alicja, Stefanowicz Piotr, Szewczuk Zbigniew
Peptides derivatized with bicyclic quaternary ammonium ionization tags. Sequencing via tandem mass spectrometry.
Setner Bartosz, Rudowska Magdalena, Klem Ewelina, Cebrat Marek, Szewczuk Zbigniew
Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI-MS.
Cydzik Marzena, Rudowska Magdalena, Stefanowicz Piotr, Szewczuk Zbigniew