Wydział Chemii

Łukasz Chrzanowski

Mariusz Dziadas

Łukasz Ławniczak

Paweł Cyplik

Wojciech Białas

Alicja Szulc

Piotr Lisiecki

Henryk H. Jeleń

2012

Bioresource Technology

111

328-335

10.1016/j.biortech.2012.01.181

Naukowa

Angielski

Artykuł

Bacterial utilization of rhamnolipids during biosurfactant-supplemented biodegradation of diesel and B20 (20% biodiesel and 80% diesel v/v) fuels was evaluated under conditions with full aeration or with nitrate and nitrite as electron acceptors. Rhamnolipid-induced changes in community dynamics were assessed by employing real-time PCR and the ddCt method for relative quantification. The experiments with rhamnolipids at 150 mg/l, approx. double critical micelle concentration (CMC) and diesel oil confirmed that rhamnolipids were readily degraded by a soil-isolated consortium of hydrocarbon degraders in all samples, under both aerobic and nitrate-reducing conditions. The presence of rhamnolipids increased the dissipation rates for B20 constituents under aerobic conditions, but did not influence the biodegradation rate of pure diesel. No effect was observed under nitrate-reducing conditions. The biodegradation of rhamnolipids did not favor the growth of any specific consortium member, which proved that the employed biosurfactant did not interfere with the microbial equilibrium during diesel/biodiesel biodegradation.

Biodegradation, Biodiesel, Diesel, Nitrate-reducing conditions, Rhamnolipids

http://dx.doi.org/10.1016/j.biortech.2012.01.181

http://www.elsevier.com