Repozytorium
Wyszukaj
Kolekcje
Inne
Modulating Copper(II) Coordination and Antimicrobial Activity: Effects of d-Amino Acid Substitution and Retro-Inverso Modification in Human Saliva MUC7 Peptide
Autorzy
Rok wydania
2025
Czasopismo
Numer woluminu
64
Strony
6365-6377
DOI
10.1021/acs.inorgchem.5c00438
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Fragments of MUC7, a salivary protein involved in nonimmune defense, arise from proteolytic cleavage in saliva and exhibit antimicrobial properties. However, their therapeutic use is limited by low stability due to further degradation. To address this, a native MUC7 fragment was modified using D-amino acids and the retro-inverso strategy. Given the role of metal ions in enhancing antimicrobial peptides, we analyzed the bioinorganic chemistry of these systems with Cu(II) and assessed their antimicrobial activity against fungal and bacterial strains. This study is the first to explore the correlation between metal binding mode, structure, stability, and antimicrobial activity of retro-inverso peptides as well as Cu(II) coordination in such systems. A combination of experimental techniques (potentiometry, mass spectrometry, UV−vis, circular dichroism, electron paramagnetic resonance, and nuclear magnetic resonance spectroscopy) and density functional theory calculations characterized their coordination chemistry. Our results demonstrate that the “standard” enantiomeric exchange and retro-inverso modifications of the MUC7 fragment have a minimal effect on the secondary structure and biological activity of the studied peptides and their Cu(II) complexes. However, these modifications significantly influence on the thermodynamic stability of studied systems.
Słowa kluczowe
Electron paramagnetic resonance spectroscopy, Ions, Ligands, Monomers, Peptides and proteins
Licencja otwartego dostępu
Licencja na prawach której można swobodnie kopiować, rozprowadzać, zmieniać i remiksować objęty prawem autorskim utwór (Utwór-przedmiot prawa autorskiego) pod warunkiem podania imienia i nazwiska autora utworu pierwotnego oraz źródła pochodzenia utworu.
Pełny tekst licencji: https://creativecommons.org/licenses/by/3.0/pl/legalcode
Adres publiczny
http://dx.doi.org/10.1021/acs.inorgchem.5c00438
Strona internetowa wydawcy
Podobne publikacje
Exploring the specificity of rationally designed peptides reconstituted from the cell-free extract of Deinococcus radiodurans toward Mn(II) and Cu(II).
Peana Massimiliano, Gumienna-Kontecka Elżbieta, Piras Francesca, Ostrowska Małgorzata, Piasta Karolina, Krzywoszyńska Karolina, Medici Serenella, Zoroddu Maria Antonietta
Bioinorganic Chemistry Meets Microbiology: Copper(II) and Zinc(II) Complexes Doing the Cha-Cha with the C-t-CCL-28 Peptide, Dancing till the End of Microbes
Szarszoń Klaudia, Baran Natalia, Śliwka Paulina, Wiloch Magdalena, Janek Tomasz, Wątły Joanna
Insights into the Chemistry, Structure, and Biological Activity of Human Salivary MUC7 Fragments and Their Cu(II) and Zn(II) Complexes
Szarszoń Klaudia, Andrä Silke, Janek Tomasz, Wątły Joanna