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Inne
Effect of multi-functional polyhydroxylated polyhedral oligomeric silsesquioxane (POSS) nanoparticles on the angiogenesis and exosome biogenesis in human umbilical vein endothelial cells (HUVECs).
Autorzy
Rok wydania
2021
Czasopismo
Numer woluminu
197
Strony
109227/1-109227/11
DOI
10.1016/j.matdes.2020.109227
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Angiogenesis, the growth of new vessels, is a vital process for tissue regeneration. Exosomes are nano-sized vesicles releasing from cells that are recognized as important soluble angiogenic factors. Although there are a lot of reports about biomedical applications of polyhedral oligomeric silsesquioxane nanoparticles (POSS NPs), the effect and mechanism of POSS NPs in angiogenesis and wound healing have not been studied until now. In this research, we have synthesized water-soluble multifunctional polyhydroxylated POSS (POH-POSS) and characterized using FT-IR, TEM, 1H NMR and 29Si NMR. We hypothesize that incubation of human endothelial cells (HUVECs) with POH-POSS NPs could contribute to the modulation of angiogenesis and exosome biogenesis. As compared to the control group, the survival rate of cells incubated with 300 ppm POH-POSS significantly increased (P < 0.05). Wound healing and migration rate of cells increased in POH-POSS treated cells (P < 0.05). Expression of genes involved in angiogenesis (VEGFR-2, Ang-1, Ang-2) and exosomes biogenesis (Alix, CD63, Rab27a, Rab27b) concurrent with miRNA-21 and miRNA-155 expression up-regulated in treated cells (P < 0.05). Our findings suggested that up-regulated angiogenesis in POH-POSS treated HUVECs is coincided with an increased exosomal secretory pathway, proposing the key role of exosomes in angiogenesis and possible biomedical application of POH-POSS.
Słowa kluczowe
POSS nanoparticles, Angiogenesis, HUVECs, Nanomedicine, Exosome, Wound healing
Licencja otwartego dostępu
Licencja na prawach której można swobodnie kopiować, rozprowadzać, zmieniać i remiksować objęty prawem autorskim utwór (Utwór-przedmiot prawa autorskiego) pod warunkiem podania imienia i nazwiska autora utworu pierwotnego oraz źródła pochodzenia utworu.
Pełny tekst licencji: https://creativecommons.org/licenses/by/3.0/pl/legalcode
Adres publiczny
http://dx.doi.org/10.1016/j.matdes.2020.109227
Strona internetowa wydawcy
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