Wydział Chemii

Kamila Stokowa-Sołtys

Karolina Kierpiec

Klaudia Szczerba

Robert Wieczorek

2023

Journal of Inorganic Biochemistry

246

112307/1-112307/13

10.1016/j.jinorgbio.2023.112307

Naukowa

Angielski

Artykuł

Outer membrane proteins of Fusobacterium nucleatum, a cancer‑leading bacteria, are considered as the factors responsible for its pathogenicity. Among them, homotrimeric autotransporter protein YadA (Yersinia adhesin A) is an important virulence factor also found in the outer membrane of pathogenic Yersinia species. In this paper, the structure and stability of certain Cu(II) complexes with YadA fragments were investigated using both, experimental and theoretical methods. Potentiometry, UV–Vis, CD, EPR, and calculations at the density functional theory (DFT) level were applied to determine the metal ion coordination sphere. Moreover, the complexes ability to DNA cleavage and reactive oxygen species (ROS) production was studied. We have shown that copper(II) complexes can cleave DNA by ¹O₂, O₂•⁻ and •OH, which are formed in the studied systems. However, the results of electrophoretic experiments revealed that complexes cleave DNA less effectively than free copper(II) ions. Therefore, the presence of studied peptides may prevent DNA from a Cu(II)-induced damage to some extent.

Fusobacterium nucleatum, YadA, Copper(II) complexes with peptides, Negatively charged complexes, Radical-induced DNA cleavage, Reactive oxygen species

CC-BY

http://dx.doi.org/10.1016/j.jinorgbio.2023.112307

http://www.elsevier.com