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Enzymatic degradation of pretreated pig bristles with crude keratinase of Bacillus cereus PCM 2849.
Autorzy
Rok wydania
2017
Czasopismo
Waste and Biomass Valorization
Numer woluminu
8
Strony
527-537
DOI
10.1007/s12649-016-9603-4
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Streszczenie
Purpose: The aim of the study was to apply and optimizethe process of bioconversion of pig bristle waste usingkeratinolytic enzymes ofBacillus cereusPCM 2849, and toevaluate the amino acid composition of the resultanthydrolysate.
Methods: Hydrolysis with concentrated culture fluid ofB.cereuswas applied for bioconversion of pig bristles, afterthermo-chemical pretreatment with sulfite. The effect ofsubstrate concentration, sulfite concentration during pre-treatment and reaction temperature on the release of aminoacids was determined using Box-Behnken design. Aminoacid composition of the obtained hydrolysate was deter-mined by HPLC. Structural condition and substructuralchanges of the residual substrate were evaluated with SEMmicroscopy and FTIR spectroscopy.
Results: The applied enzymatic preparation for bristlebiodegradation was verified to contain multiple proteasesof a wide molecular weight range. A regression model wasdeveloped, in which influential parameters were: lineareffect of substrate concentration, followed by quadraticeffects of reaction temperature, substrate concentration andpretreatment. Optimum reaction conditions were alsodetermined. The resultant hydrolysate was rich in bran-ched-chain amino acids. Residual substrate was detrioratedand sulfitolytic cleavage of disulfides and alteration ofprotein secondary structures was confirmed.
Conclusions: Application ofB. cereuscrude keratinaseallowed for partial hydrolysis of pig bristles, preceded bysulfitolytic pretreatment. A regression model was built todescribe the process of hydrolysis to release free aminoacids, at constant enzyme load. Hydrolysis in given con-ditions allowed to obtain hydrolysate rich in branchedchain amino acids. The presented process poses an alter-nate way of management over pig bristles, a hard-to-de-grade keratinous waste.
Słowa kluczowe
Pig bristles, Biodegradation, Bacillus cereus, Keratinase, Box-Behnken design, Hydrolysis
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Pełny tekst licencji: https://creativecommons.org/licenses/by/3.0/pl/legalcode
Adres publiczny
http://dx.doi.org/10.1007/s12649-016-9603-4
Strona internetowa wydawcy
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