Wydział Chemii

Marta Kuć

Katarzyna Cieślik-Boczula

Maria Rospenk

2023

Wiadomości Chemiczne

77

647-686

10.53584/WIADCHEM.2023.07.2

Naukowa

Polski

Artykuł

Biomembranes,which are the structural and functional basis of the cells of all living organisms, have been an extremely interesting research object for biology and chemistry scientist sforyears. The multitude of elements constituting the component sof natural lipid membranes, however,is associated with interpretation difficulties regarding the nature of the processes taking place in them. A useful research object that is a model of bilamellar biosystems with a significantly simplified composition  and at the same time retaining properties that can be a reference point in relation to natural membranes are lipid membranes in the form of one orseveral component liposomes.It is precisely such systems built of molecules of dipalmitoyl phosphatidylcholine(DPPC)or dipalmitoyl phosphatidyl glycerol(DPPG),and analogoussystems with the addition of cholesterol(Chol),that were the subject of research in this work. Near-infrared (NIR) vibrational spectroscopy provides a suitable method for the study of the hydrated samples. In most cases it can be alternatively adopted instead of commonly used mid-infrared (MIR) vibrational spectroscopy. This technique was applied for the first time to identify the spectral changes associated with the conformational changes in the hydrophobic region of model lipid bilayers. Trans/gauche isomerization of CH2 groups of lipid hydrocarbon chains is accompanied by characteristic changes in spectral parameters of both νas,s CH2 bands and their first overtones (2νas,s CH2). The heating of all types of analyzed liposomes results in main phase transition (Tm) accompanied by trans to gauche isomerization of CH2 groups of lipid hydrocarbon chains. The NIR-spectroscopy was able to describe in proper way (similar to MIR results) the character of Tm in studied bilayers.

Błony biologiczne. Budowa i funkcje błon biologicznych. Właściwości błon biologicznych. Liposomy jako modele błon biologicznych. Przejścia fazowe modelowych dwuwarstw lipidowych. Metody badawcze układów bilamelarnych. Wspomagana analizą chemometryczną (PCA) spektroskopia FTIR wzakresie NIR jako alternatywna metoda w badaniu przejść fazowych modelowych układów błonowych w zakresie MIR.

lipid bilayers, liposome, main phase transition, Mid - infrared vibrational spectroscopy (MIR), Near - infrared vibrational spectroscopy (NIR), Principal Component Analysis (PCA)

dwuwarstwa lipidowa, liposom, główne przejście fazowe, spektroskopia oscylacyjna w zakresie średniej podczerwieni (MIR), spektroskopia oscylacyjna w zakresie bliskiej podczerwieni (NIR), Analiza Składników Głównych (PCA)

OTHER

https://ptchem.pl/storage/pages/July2023/Boczula.pdf\