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Inne
Identyfication of tryptic podocin peptide in the feline urine sediments using LC-MS/MRM method.
Autorzy
Rok wydania
2019
Czasopismo
International Journal of Mass Spectrometry
Numer woluminu
444
Strony
116174/1-116174/6
DOI
10.1016/j.ijms.2019.116174
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Chronic kidney disease (CKD) as one of the major public health problem itself and also strongly associated with cardiovascular diseases is a major mortality factor in humans, as well as in dogs and cats. Recently, urinary podocin has been identified as potential CKD biomarker. However, still the sensitive and straightforward method of urinary podocin detection and identification is needed. Here we report our investigations on the applicability of liquid chromatography coupled with tandem mass spectrometry in the analysis of feline urinary podocytes, based on the identification of podocin tryptic peptide with the 222AVQFLVQTTMK235 sequence. This peptide was chosen due to the best response of all of the model podocin tryptic peptides in a feline urine sediment tryptic digest as analyzed by LC-MS/MS. Performed study revealed the presence of the model podocin tryptic peptide in the analyzed feline urine sediments with confirmed chronic kidney disease. The obtained results clearly confirmed the applicability of the proposed methodology in the analysis of podocin in feline urine sediments.
Słowa kluczowe
LC-MS, Multiple reaction monitoring, Podocin, Chronic kidney diseases, Proteinuria
Licencja otwartego dostępu
Licencja na prawach której można swobodnie kopiować, rozprowadzać, zmieniać i remiksować objęty prawem autorskim utwór (Utwór-przedmiot prawa autorskiego) pod warunkiem podania imienia i nazwiska autora utworu pierwotnego oraz źródła pochodzenia utworu.
Pełny tekst licencji: https://creativecommons.org/licenses/by/3.0/pl/legalcode
Adres publiczny
http://dx.doi.org/10.1016/j.ijms.2019.116174
Strona internetowa wydawcy
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