Wydział Chemii

Remigiusz Bąchor

Andrzej Konieczny

Zbigniew Szewczuk

2020

Molecules

25

1514/1-1514/10

10.3390/molecules25071514

Naukowa

Angielski

Artykuł

Kidneys play a crucial role in maintaining metabolic homeostasis in a body. Serum
creatinine concentration is a simple test used as an indicator of renal function. One of the known ways
of quantifying creatinine concentration is the liquid chromatography-mass spectrometry (LC-MS)
method, using an isotopically labeled analog of creatinine as an internal standard. Unfortunately,
such isotope-labeled analogs are expensive and their synthesis is complex. Here we demonstrate
a facile preparation of deuterated analogues of creatinine, via the H/D exchange of hydrogens
located at the -carbon (-C) of the N-methylated amino acid part, under basic conditions. The
stability of retrieved isotopologues was analyzed under both neutral or acidic conditions, and the
results revealed that the introduced deuterons do not undergo back-exchange. In addition, the
coelution of deuterated and non-deuterated forms under acidic and neutral conditions was observed.
The prepared isotopologues were successfully applied in the quantitative LC-MS analysis of urine
samples, and the results demonstrated that the presented strategy is novel and inexpensive, and that
the quantification correlates with the commonly used Jae test method.

creatinine, hydrogen-deuterium exchange, isotope dilution, liquid chromatography ‐ mass spectrometry

CC-BY

http://dx.doi.org/10.3390/molecules25071514

http://www.mdpi.com/journal/metals