Repozytorium

Some aspect of the interactions of adriamycin with human serum albumin.

Autorzy

Lilianna Trynda-Lemiesz

Henryk Kozłowski

Rok wydania

1996

Czasopismo

Bioorganic and Medicinal Chemistry

Numer woluminu

4

Strony

1709-1713

DOI

10.1016/0968-0896(96)00162-9

Kolekcja

Naukowa

Język

Angielski

Typ publikacji

Artykuł

Streszczenie

The interaction of adriamycin with human serum albumin (HSA) has been studied by absorption, CD, fluorescence spectroscopy, and quantitative precipitating HSA-antibody test. Our results demonstrate that adriamycin react with HSA and the binding to the protein molecule has a very distinct influence on the stability of ADR in aqueous solutions. The drug molecule binds protein as a monomer. The structural studies have shown the conformational change of HSA modified by adriamycin. The binding of ADR lowers the helicity of the native protein of ca. 15% and ca. 10% in the case of acHSA. The quantitative precipitating test supports distinct changes in the conformation upon ADR binding that decreases the ability of HSA to precipitate with its antibody.

Adres publiczny

https://doi.org/10.1016/0968-0896(96)00162-9

Strona internetowa wydawcy

http://www.elsevier.com

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