Wydział Chemii

Anna Rola

Paulina Potok

Magdalena Mos

Elżbieta Gumienna-Kontecka

Sławomir Potocki

2022

Inorganic Chemistry

61

14333-14343

10.1021/acs.inorgchem.2c02080

Naukowa

Angielski

Artykuł

Infections caused by Candida species are becoming seriously dangerous and difficult to cure due to their sophisticated mechanisms of resistance. The host organism defends itself from the invader, e.g., by increasing the concentration of metal ions. Therefore, there is a need to understand the overall mechanisms of metal homeostasis in Candida species. One of them is associated with AMT1, an important virulence factor derived from Candida glabrata, and another with MAC1, present in Candida albicans. Both of the proteins possess a homologous Cys/His-rich domain. In our studies, we have chosen two model peptides, L680 (Ac-₁₀ACMECVRGHRSSSCKHHE₂₇-NH₂, MAC1, Candida albicans) and L681 (Ac-₁₀ACDSCIKSHKAAQCEHNDR₂₈-NH₂, AMT1, Candida glabrata), to analyze and compare the properties of their complexes with Zn(II) and Cd(II). We studied the stoichiometry, thermodynamic stability, and spectroscopic parameters of the complexes in a wide pH range. When competing for the metal ion in the equimolar mixture of two ligands and Cd(II)/Zn(II), L680 forms more stable complexes with Cd(II) while L681 forms more stable complexes with Zn(II) in a wide pH range. Interestingly, a Glu residue was responsible for the additional stability of Cd(II)-L680. Despite a number of scientific reports suggesting Cd(II) as an efficient surrogate of Zn(II), we showed significant differences between the Zn(II) and Cd(II) complexes of the studied peptides.

CC-BY

http://dx.doi.org/10.1021/acs.inorgchem.2c02080

https://www.acs.org/content/acs/en.html