Wydział Chemii

Monika K. Lesiów

Bartosz Kwiatkowski

Piotr Pietrzyk

Agnieszka Kyzioł

Krzysztof Rolka

Urszula K. Komarnicka

2025

Inorganic Chemistry

64

15502-15517

10.1021/acs.inorgchem.5c01747

Naukowa

Angielski

Artykuł

FomA is a major outer membrane protein of Fusobacterium nucleatum (Fn) overexpressed in colorectal cancer (CRC) tissues, where increased levels of Cu ions together with reactive oxygen species (ROS) were noted. In this comparative study, the connection between Fn surrounded by copper ions and the ROS overgeneration leading to the development of CRC is examined. Our study focused on a model peptide Ac-KGHGNGEEGTPTVHNEYH-NH₂ (6L) derived from the FomA reflecting a fragment of the protein loop no. 4, exposed to the external environment, which facilitates the binding of Cu ions. The coordination studies (potentiometric titration, UV–Vis, CD, EPR) showed that the 6L bound Cu(II) and formed mono-, di-, and trinuclear complexes depending on the solution pH. The ability to generate ROS by promoting the Cu(III)/Cu(II)/Cu(I) redox cycle was proven by CV and EPR techniques. We also confirmed that upon adding Asc, the 6L was fragmented and after copper coordination, the ROS production increased (e.g.,^•OH, ¹O₂, O₂^•-), leading to DNA damage. Stimulation of model mouse colon carcinoma cells by Cu(II) complex with 6L demonstrated an abundant cellular ROS production, resulting in pronounced lipid peroxidation. Hypothetically, this action mode leads to the damage of colon cells and triggers carcinogenesis processes.

Free radicals, Hydroxyls, Ions, Ligands, Peptides and proteins

CC-BY

http://dx.doi.org/10.1021/acs.inorgchem.5c01747

https://www.acs.org/content/acs/en.html