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Inne
Microwave-assisted 18O labeling of Fmoc-protected amino acids.
Autorzy
Rok wydania
2014
Czasopismo
Numer woluminu
20
Strony
896-900
DOI
10.1002/psc.2682
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Recently, there has been an increased interest in isotopical labeling of peptides. Although there are several techniques allowing for a complete labeling of all carboxyl groups in peptides, regioselective labeling would be beneficial in many situations. Such labeling requires the use of 18O‐labeled Fmoc amino acids. We have designed a method for such labeling that is an improvement on a technique proposed earlier. The new procedure is suitable for microscale synthesis and could be used in peptide and proteomics laboratories. Although for the majority of tested amino acids our method gives good labeling efficiency, it is time consuming. Therefore, we have decided to use microwave‐assisted procedure. This approach resulted in reduction of reaction time to 15 min and increased reaction efficiency.
Słowa kluczowe
18 O labeling, quantitative proteomics, peptide synthesis, stable isotopes, microwave-assisted labeling
Adres publiczny
http://dx.doi.org/10.1002/psc.2682
Strona internetowa wydawcy
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