Wydział Chemii

D. Balenci

G. Bonechi

N. D'Amelio

Elena Gaggelli

Nicola Gaggelli

E. Molteni

Gianni Valensin

Wojciech Szczepanik

Magdalena P. Dziuba

G. Święcicki

Małgorzata Jeżowska-Bojczuk

2009

Dalton Transactions

1123-1130

10.1039/b815046j

Naukowa

Angielski

Artykuł

The interaction of apramycin with copper at different pH values was investigated by potentiometric titrations and EPR, UV-vis and CD spectroscopic techniques. The Cu(II)-apramycin complex prevailing at pH 6.5 was further characterized by NMR spectroscopy. Metal-proton distances derived from paramagnetic relaxation enhancements were used as restraints in a conformational search procedure in order to define the structure of the complex. Longitudinal relaxation rates were measured with the IR-COSY pulse sequence, thus solving the problems due to signal overlap. At pH 6.5 apramycin binds copper(II) with a 2 : 1 stoichiometry, through the vicinal hydroxyl and deprotonated amino groups of ring III. Plasmid DNA electrophoresis showed that the Cu(II)-apramycin complex is more active than free Cu(II) in generating strand breakages. Interestingly, this complex in the presence of ascorbic acid damages DNA with a higher yield than in the presence of H₂O₂.

https://doi.org/10.1039/b815046j

https://www.rsc.org/