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Electron capture dissociation mass spectrometric analysis of lysine-phosphorylated peptides.
Autorzy
Rok wydania
2010
Czasopismo
Numer woluminu
30
Strony
433-443
DOI
10.1042/BSR20090167
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Streszczenie
Phosphorylation of proteins is an essential signalling mechanism in eukaryotic and prokaryotic cells. Although Nphosphorylation of basic amino acid is known for its importance in biological systems, it is still poorly explored in terms of products and mechanisms. In the present study, two MS fragmentation methods, ECD (electron-capture dissociation) and CID (collision-induced dissociation), were tested as tools for analysis of N-phosphorylation of three model peptides, RKRSRAE, RKRARKE and PLSRTLSVAAKK. The peptides were phosphorylated by reaction with monopotassium phosphoramidate. The results were confirmed by 1H NMR and 31P NMR studies. The ECD method was found useful for the localization of phosphorylation sites in unstable lysine-phosphorylated peptides. Its main advantage is a significant reduction of the neutral losses related to the phosphoramidate moiety. Moreover, the results indicate that the ECD–MS may be useful for analysis of regioselectivity of the N-phosphorylation reaction. Stabilities of the obtained lysine-phosphorylated peptides under various conditions were also tested.
Słowa kluczowe
electron capture dissociation, phosphorylation, phospholysine, post-translational modification
Licencja otwartego dostępu
Licencja ta pozwala na kopiowanie, zmienianie, remiksowanie, rozprowadzanie, przedstawienie i wykonywanie utworu jedynie w celach niekomercyjnych. Warunek ten nie obejmuje jednak utworów zależnych (mogą zostać objęte inną licencją).
Pełny tekst licencji: https://creativecommons.org/licenses/by-nc/4.0/legalcode
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