Repozytorium
Wyszukaj
Kolekcje
Inne
The pro-apoptotic action of the peptide hormone Neb--colloostatin on insect haemocytes.
Autorzy
Rok wydania
2012
Czasopismo
Journal of Experimental Biology
Numer woluminu
215
Strony
4308-4313
DOI
10.1242/jeb.072579
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
The gonadoinhibitory peptide hormone Neb-colloostatin was first isolated from ovaries of the flesh fly Neobellieria bullata. This 19-mer peptide is thought to be a cleaved product of a collagen-like precursor molecule that is formed during remodelling of the extracellular matrix. In this study, we report that upon injection of picomolar and nanomolar doses, this peptide exerts a pro-apoptotic action on haemocytes of Tenebrio molitor adults, as visualized by changes in morphology and viability. The F-actin cytoskeleton was found to aggregate into distinctive patches. This may be responsible for the observed inhibition of adhesion of haemocytes and for the stimulation of filopodia formation. However, Neb-colloostatin injection did not induce the formation of autophagic vacuoles. Our results suggest that physiological concentrations of Neb-colloostatin play an important role in controlling the quantity and activity of haemocytes in insect haemolymph. They also suggest that during periods in which Neb-colloostatin is released, this peptide may cause a weakening of the insects’ immune system. This is the first report that exposure to a peptide hormone causes apoptosis in insect haemocytes.
Słowa kluczowe
insects, haemocytes, Neb-colloostatin, Apoptosis
Adres publiczny
Podobne publikacje
The pro-apoptotic action of new analogs of the insect gonadoinhibiting peptide Neb-colloostatin: synthesis and structure-activity studies.
Kuczer Mariola, Czarniewska Elżbieta, Rosiński Grzegorz, Lisowski Marek
Copper(II) complexes of Neb-colloostatin and of (P4A) analogue stability structure apoptosis.
Kuczer Mariola, Matusiak Agnieszka, Czarniewska Elżbieta, Rosiński Grzegorz, Kowalik-Jankowska Teresa
The natural insect peptide Neb-colloostatin induces ovarian atresia and apoptosis in the mealworm Tenebrio molitor.
Czarniewska Elżbieta, Rosiński Grzegorz, Gabała Elżbieta, Kuczer Mariola