Wydział Chemii

Urszula K. Komarnicka

Radosław Starosta

Michał Płotek

Rodrigo F. M. de Almeida

Małgorzata Jeżowska-Bojczuk

Agnieszka Kyzioł

2016

Dalton Transactions

45

5052-5063

10.1039/C5DT04011F

Naukowa

Angielski

Artykuł

In this paper we present a first insight into the cytotoxic action mode of copper(I) iodide or copper(I) thiocyanate complexes with a phosphine derivative of sparfloxacin (a 3^rdgeneration fluoroquinolone antibiotic agent) and 2,9-dimethyl-1,10-phenanthroline or 2,2′-biquinoline as auxiliary ligands. Thein vitrocytotoxic activity of the new complexes was tested against two cancer cell lines (CT26 - mouse colon carcinoma and A549 - human lung adenocarcinoma). An ICP-MS study revealed a marked time-dependent intracellular copper accumulation of the tested compounds. In addition, confocal microscopy imaging showed accumulation of the complexes inside whole cells and their emission of blue light. The complexes generate reactive oxygen species in the cancer cells, which was examined by using two different fluorescent probes. Moreover, (i) DNA intercalation studied by luminescence spectroscopy, circular dichroism and molecular docking, and (ii) plasmid DNA damage also demonstrate their significant cytotoxicity. All these observed biological effects contribute to the induction of apoptosis, observed at a great predominance.

http://dx.doi.org/10.1039/C5DT04011F

https://www.rsc.org/