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Inne
Impact of histidine spacing on modified polyhistidine tag : metal ion interactions.
Autorzy
Rok wydania
2018
Czasopismo
Numer woluminu
472
Strony
119-126
DOI
10.1016/j.ica.2017.06.053
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Histidine rich sequences are chosen both by nature and by molecular biologists due to their high affinity towards metal ions. In this work, we examine the affinity and binding modes of Cu2+, Ni2+ and Zn2+ towards two histidine tags, the common His6-tag (Ac-HHHHHH-NH2) and its modified sequence, which also contains six histidines, but separated with two alanineresidues (Ac-HAAHAAHAAHAAHAAHAA-NH2). The spatial separation of histidines has an important impact on its coordination properties. Cu2+ and Ni2+ complexes with Ac-HHHHHH-NH2 are more stable than those with Ac-HAAHAAHAAHAAHAAHAA-NH2; the contrary is observed for Zn2+. In a narrow range of pH, Cu2+-Ac-HHHHHH-NH2 and Ni2+-Ac-HHHHHH-NH2 can even compete with the albumin-like binding of the respective metals.
Adres publiczny
https://doi.org/10.1016/j.ica.2017.06.053
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