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Unravelling the Mystery of COVID‐19 Pathogenesis: Spike Protein and Cu Can Synergize to Trigger ROS Production
Autorzy
Rok wydania
2023
Czasopismo
Numer woluminu
29
Strony
e202301530/1-e202301530/11
DOI
10.1002/chem.202301530
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
The COVID-19 pandemic has had a devastating impact on global health, highlighting the need to understand how the SARS-CoV-2 virus damages the lungs in order to develop effective treatments. Recent research has shown that patients with COVID-19 experience severe oxidative damage to various biomolecules. We propose that the overproduction of reactive oxygen species (ROS) in SARS-CoV-2 infection involves an interaction between copper ions and the virus‘s spike protein. We tested two peptide fragments, Ac-ELDKYFKNH-NH2 (L1) and Ac-WSHPQFEK-NH2 (L2), derived from the spike protein of the Wuhan strain and the β variant, respectively, and found that they bind Cu(II) ions and form a three-nitrogen complexes at lung pH. Our research demonstrates that these complexes trigger the overproduction of ROS, which can break both DNA strands and transform DNA into its linear form. Using A549 cells, we demonstrated that ROS overproduction occurs in the mitochondria, not in the cytoplasm. Our findings highlight the importance of the interaction between copper ions and the virus‘s spike protein in the development of lung damage and may aid in the development of therapeutic procedures.
Słowa kluczowe
COVID-19, copper, DNA damage, ROS, SARS-CoV-2, spike protein
Adres publiczny
http://dx.doi.org/10.1002/chem.202301530
Strona internetowa wydawcy
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