Wydział Chemii

Terézia Vojtylová

Véra Hamplová

Zbigniew Galewski

Izabela Korbecka

David Sýkora

2017

Journal of Separation Science

40

1465-1469

10.1002/jssc.201601386

Naukowa

Angielski

Artykuł

Chiral high-performance liquid chromatography separation of two recently synthesized liquid crystalline materials C1 and C2 was studied in the reversed-phase mode. Both materials have an azo-moiety and one chiral center in their molecular structures. They were available in racemic and pure S forms. For the enantiomeric separations, a Chiralpak AY-RH stationary phase based on amylose tris(5-chloro-2-methylphenylcarbamate) coated on 5 μm silica was used. The compounds were analyzed in both of their possible forms, the more thermodynamically stable E form and the labile Z form. The conditions and time scale of the UV-induced E to Z transition were briefly evaluated. Under the optimized conditions, we were able to baseline separate S and R enantiomers of both of the studied materials not only in their E forms, but also in their Z forms. In comparison to the separation in the normal-phase mode, which we have reported recently, the resolution in the reversed-phase mode is significantly better. Interestingly, peak reversal was noticed for the S and R enantiomers when the separation was carried out with E versus Z forms of both compounds.

azobenzene, chiral separation, high-performance liquid chromatography, liquid-crystalline materials, photo-induced isomerization

http://dx.doi.org/10.1002/jssc.201601386

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