Repozytorium
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Kolekcje
Inne
Synthesis and structure elucidation of a cobalt(II) complex as topoisomerase I inhibitor:In vitro DNA binding, nuclease and RBC hemolysis.
Autorzy
Rok wydania
2014
Czasopismo
European Journal of Medicinal Chemistry
Numer woluminu
74
Strony
683-693
DOI
10.1016/j.ejmech.2013.10.025
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Metal-based cancer chemotherapeutic agent of the type Co(II) complex [Co(mpca)2]·H2O (1), where, Hmpca = 9-methyl-[1,10]phenanthroline-2-carboxylic acid was synthesized and characterized by various spectroscopic and analytical techniques and further authenticated by single crystal X-ray diffraction methods. In vitro DNA binding studies of complex 1 with CT DNA was carried out by several biophysical techniques in accordance with molecular docking technique which revealed that 1 binds to DNA via intercalation mode having GC-rich sequences. Complex 1 cleaves pBR322 DNA via hydrolytic pathway (validated by T4 DNA ligase assay). Furthermore, complex 1 exhibits significant inhibitory effects on the catalytic activity of Topo-I at 25 μM concentration and further validated by molecular docking studies.
Słowa kluczowe
CT DNA binding, pBR322 DNA cleavage, T4 DNA ligase assay, Topo I inhibition activity, Molecular docking
Adres publiczny
http://dx.doi.org/10.1016/j.ejmech.2013.10.025
Strona internetowa wydawcy
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