Repozytorium
Wyszukaj
Kolekcje
Inne
Conotoxin-derived biomimetic coiled cone-shaped peptide as ligand for selective nanodelivery to norepinephrine transporter-expressing neuroblastoma cells.
Autorzy
Rok wydania
2022
Czasopismo
Numer woluminu
27
Strony
101410/1-101410/14
DOI
10.1016/j.apmt.2022.101410
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Selective drug delivery is an emerging powerful strategy, which promises efficient and safer neuroblastoma treatment in contrast to conventional therapeutic modalities. Herein, we present a newly designed apoferritin nanovehicle loaded with ellipticine and decorated with conotoxin-derived biomimetic peptide YKL-6 (sequence: YKLYKLYKLYKLYKLYKLC) to target the norepinephrine transporter (hNET), which is highly expressed on neuroblastoma cells membrane. The peptide forms a coiled cone-shape forming anti-parallel beta sheets in aggregation in vitro. Molecular docking analysis suggests electrostatic interaction mediated by lysine residues against negatively charged residues of the hNET. The assembled nanovehicles show optimal biocompatibility in healthy epithelial cells and ability to deliver ellipticine into neuroblastoma cells (UKF-NB-4 and SH-SY5Y). In our analyses, we have excluded hNET-mediated endocytosis as an uptake mechanism and demonstrated the ellipticine-triggered anti-proliferative mechanisms in neuroblastoma cells via downregulation of Bcl-2, survivin and N-myc. Finally, we present a selective inhibition of neuroblastoma growth and metastasis in vivo in an ex ovo and in ovo chicken chorioallantoic membrane assay. Overall, these findings demonstrate a great leap forward in the design of biomimetic homing peptides towards possible application in neuroblastoma nanomedicine.
Słowa kluczowe
Apoferritin, Biomimetic peptides, Drug delivery, Neuroblastoma, Norepinephrine transporter, Targeted therapy
Adres publiczny
http://dx.doi.org/10.1016/j.apmt.2022.101410
Strona internetowa wydawcy
Podobne publikacje
Norepinephrine transporter-derived homing peptides enable rapid endocytosis of drug delivery nanovehicles into neuroblastoma cells.
Haddad Yazan, Charousova Marketa, Zivotska Hana, Splichal Zbynek, Rodrigo Miguel Angel Merlos, Michalkova Hana, Krizkova Sona, Tesarova Barbora, Richtera Lukas, Vitek Petr, Stokowa-Sołtys Kamila, Hynek David, Milosavljevic Vedran, Rex Simona, Heger Zbynek
Recent advances in carbohydrate-based paclitaxel delivery systems
Ramezanpour Azar, Ansari Legha, Rahimkhoei Vahid, Sharifi Sina, Bigham Ashkan, Lighvan Zohre Mehri, Rezaie Jafar, Szafert Sławomir, Mahdavinia GholamReza, Akbari Ali, Jabbari Esmaiel
Polymeric micelle-mediated delivery of half-sandwich ruthenium(II) complexes with phosphanes derived from fluoroloquinolones for lung adenocarcinoma treatment.
Kołoczek Przemysław, Skórska-Stania Agnieszka, Cierniak Agnieszka, Sebastian Victor, Komarnicka Urszula K., Płotek Michał, Kyzioł Agnieszka