Repozytorium

Hexaamminecobalt(III) - probing metal ion binding sites in nucleic acids by NMR spectroscopy.

Autorzy

Magdalena Rowińska-Żyrek

Miriam Skilandat

Roland K. O. Sigel

Rok wydania

2013

Czasopismo

Zeitschrift für Anorganische und Allgemeine Chemie

Numer woluminu

639

Strony

1313-1320

DOI

10.1002/zaac.201300123

Kolekcja

Naukowa

Język

Angielski

Typ publikacji

Artykuł

Streszczenie

Hexaamminecobalt(III), an octahedral, inert metal ion complex, has lately gained increasing attention of structural biologists and bioinorganic chemists due to its use in structure determination of nucleic acids. This complex mimics outer‐sphere binding events of the physiologically relevant magnesium(II)hexaaqua ion; hexaamminecobalt(III) often finds usage either in NMR spectroscopy, where cross‐peaks between the complex ammines and the nucleic acid protons near the binding site are observed, in X‐ray spectroscopy as heavy metal derivative for phasing, or in other techniques. In this review, we discuss the basic hexaamminecobalt(III) binding modes and give an overview on the most recent findings on [Co(NH3)6]Cl3–nucleic acid complexes. The various techniques that are applied in combination with this complex are mentioned and briefly summarized. Special attention is given to the application of [Co(NH3)6]Cl3 in nuclear magnetic resonance spectroscopy of nucleic acids, where it is used to reveal potential outer‐sphere magnesium binding sites.

Słowa kluczowe

Hexaamminecobalt(III), Nucleic acids, NMR spectroscopy, Metal ions

Adres publiczny

http://dx.doi.org/10.1002/zaac.201300123

Strona internetowa wydawcy

onlinelibrary.wiley.com

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