Wydział Chemii

Edyta Wysokińska

Jakub Cichos

Ewa Zioło

Artur Bednarkiewicz

Leon Strządała

Mirosław Karbowiak

Dariusz Hreniak

Wojciech Kałas

2016

Toxicology in Vitro

32

16-25

10.1016/j.tiv.2015.11.021

Naukowa

Angielski

Artykuł

The lanthanide nano-compounds are well suited to serve as fluorescent and magnetic contrast agents and luminescent labels. Although they are considered as promising materials for bio-imaging and bio-sensors in vivo or in vitro, the amount of data is still insufficient for deep understanding the toxicity of these nanomaterials. This knowledge is of great importance in the light of growing use of the biofunctionalized nanoparticles, which raises some questions about safety of these materials. Despite lanthanide-doped NaGdF₄nanocrystals are considered as non-toxic, here we present the data showing the fatal effect of newly synthetized NaGdF₄:Yb³⁺:Er³⁺on chosen types of cells. Our studies were performed on two cell lines NIH3T3 fibroblasts, and RAW264.7 macrophages. Cytotoxic properties of NaGdF₄:Yb³⁺:Er³⁺nanoparticles and their biological effects were studied by assessing cell culture viability (MTS), proliferation and apoptosis. Bare NaGdF₄:Yb³⁺:Er³⁺nanocrystals were cytotoxic and induced apoptosis of both NIH3T3 and RAW264.7 cells. Their cytotoxicity was reduced by PEGylation, at the expense of minimizing direct interactions between the compound and the cell. On the other hand, coating with silica reduced cell death induced by Yb³⁺:Er³⁺codoped NaGdF₄nanocrystals (but proliferation was still inhibited). The NH₂-modified silica coated nanoparticles were clearly less cytotoxic than pristine nanoparticles, which suggests that both, silica and PEG coatings are reasonable approaches to decrease cytotoxicity of the nanocrystal labels. The silica and PEG shell, should also enable and simplify further bio-functionalization of these luminescent labels.

Lanthanide-doped nanocrystals, NaGdF4 nanocrystals, Nanomaterial toxicity, cytotoxicity, Apoptosis, proliferation, Fibroblasts, Macrophages

http://dx.doi.org/10.1016/j.tiv.2015.11.021

http://www.elsevier.com