Repozytorium
Wyszukaj
Kolekcje
Inne
A novel branched TAT47-57 peptide for selective Ni2+ introduction into the human fibrosarcoma cell nucleus.
Autorzy
Rok wydania
2015
Czasopismo
Numer woluminu
7
Strony
1155-1162
DOI
10.1039/c5mt00021a
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
A TAT47–57 peptide was modified on the N-terminus by elongation with a 2,3-diaminopropionic acid residue and then by coupling of two histidine residues on its N-atoms. This branched peptide could bind to Ni under physiological conditions as a 1 : 1 complex. We demonstrated that the complex was quantitatively taken up by human fibrosarcoma cells, in contrast to Ni2+ ions. Ni localization (especially at the nuclei) was confirmed by imaging using both scanning X-ray fluorescence microscopy and Newport Green fluorescence. A competitive assay with Newport Green showed that the latter displaced the peptide ligand from the Ni-complex. Ni2+ delivered as a complex with the designed peptide induced substantially more DNA damage than when introduced as a free ion. The availability of such a construct opens up the way to investigate the importance of the nucleus as a target for the cytotoxicity, genotoxicity or carcinogenicity of Ni2+.
Adres publiczny
http://dx.doi.org/10.1039/c5mt00021a
Strona internetowa wydawcy
Strona internetowa wydawcy
Podobne publikacje
Optical spectra and crystal field calculation for SrB4O7:Sm2+.
Solarz Piotr, Karbowiak Mirosław, Głowacki Michał, Berkowski Marek, Diduszko Ryszard, Ryba-Romanowski Witold
Copper complexes with N-allylisoquinolinium halides: synthesis and crystal structures of [C9H7N(C3H5)]2CuIICl2.86Br1.14, [C9H7N(C3H5)CuIBr2]·H2O, and [C9H7N(C3H5)CuIbr2].
Pavlyuk A. V., Kinzhybalo Vasyl V., Lis Tadeusz, Mys’kiv M. G.