Repozytorium

Impact of Cu2+ ions on the structure of colistin and cell-free system nucleic acid degradation.

Autorzy

Kamila Stokowa-Sołtys

Aleksandra Kasprowicz

Jan Wrzesiński

Jerzy Ciesiołka

Nicola Gaggelli

Elena Gaggelli

Gianni Valensin

Małgorzata Jeżowska-Bojczuk

Rok wydania

2015

Czasopismo

Journal of Inorganic Biochemistry

Numer woluminu

151

Strony

67-74

DOI

10.1016/j.jinorgbio.2015.05.011

Kolekcja

Naukowa

Język

Angielski

Typ publikacji

Artykuł

Streszczenie

Colistin and transition metal ions are commonly used as feed additives for livestock animals. This work presents the results of an analysis of combined potentiometric and spectroscopic (UV–vis, EPR, CD, NMR) data which lead to conclude that colistin is able to effectively chelate copper(II) ions. In cell-free system the oxidative activity of the complex manifests itself in the plasmid DNA destruction with simultaneous generation of reactive OH species, when accompanied by hydrogen peroxide or ascorbic acid. The degradation of RNA occurs most likely via a hydrolytic mechanism not only for complexed compound but also colistin alone. Therefore, huge amounts of the used antibiotic for nontherapeutic purposes might have a potential influence on livestock health.

Słowa kluczowe

colistin, copper(II) complex, DNA and RNA degradation, Oxidative and hydrolytic properties

Adres publiczny

http://dx.doi.org/10.1016/j.jinorgbio.2015.05.011

Strona internetowa wydawcy

http://www.elsevier.com

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