Wydział Chemii

Mykhaylo Losytskyy

Nina Chornenka

Serhii Vakarov

Samuel M. Meier-Menches

Christopher Gerner

Sławomir Potocki

Vladimir B. Arion

Elżbieta Gumienna-Kontecka

Yan Voloshin

Vladyslava Kovalska

2020

Biomolecules

10

1602/1-1602/16

10.3390/biom10121602

Naukowa

Angielski

Artykuł

Recognition of elements of protein tertiary structure is crucial for biotechnological and biomedical tasks; this makes the development of optical sensors for certain protein surface elements important. Herein, we demonstrated the ability of iron(II) clathrochelates (1–3) functionalized with mono-, di- and hexa-carboxyalkylsulfide to induce selective circular dichroism (CD) response upon binding to globular proteins. Thus, inherently CD-silent clathrochelates revealed selective inducing of CD spectra when binding to human serum albumin (HSA) (1, 2), beta-lactoglobuline (2) and bovine serum albumin (BSA) (3). Hence, functionalization of iron(II) clathrochelates with the carboxyalkylsulfide group appears to be a promising tool for the design of CD-probes sensitive to certain surface elements of proteins tertiary structure. Additionally, interaction of 1–3 with proteins was also studied by isothermal titration calorimetry, protein fluorescence quenching, electrospray ionization mass spectrometry (ESI-MS) and computer simulations. Formation of both 1:1 and 1:2 assemblies of HSA with 1–3 was evidenced by ESI-MS. A protein fluorescence quenching study suggests that 3 binds with both BSA and HSA via the sites close to Trp residues. Molecular docking calculations indicate that for both BSA and HSA, binding of 3 to Site I and to an “additional site” is more favorable energetically than binding to Site II.

iron(II) clathrochelates, globular proteins, albumins, CD reporters, ITC, ESI-TOF MS, fluorescence quenching

CC-BY

http://dx.doi.org/10.3390/biom10121602

http://www.mdpi.com/journal/metals