Wydział Chemii

S. Streiff

N. Ribeiro

Z. Wu

Elżbieta Gumienna-Kontecka

M. Elhabiri

A.-M. Albrecht-Gary

G. Ourisson

Y. Nakatani

2007

Chemistry and Biology

14

313-319

10.1016/j.chembiol.2006.11.017

Naukowa

Angielski

Artykuł

Polyprenyl phosphates, as well as polyprenyl alcohols bearing different isopentenyl C₅ units, have been synthesized. The pH range of spontaneous vesicle formation of polyprenyl phosphates with or without polyprenyl alcohols was defined by fluorescence microscopy. A variety of the acyclic or monocyclic polyprenyl phosphates studied formed stable vesicles in water over a wide range of pHs, and the addition of polyprenyl alcohols allowed the vesicle formation of polyprenyl phosphates at higher pHs. Osmotic swelling of a suspension of unilamellar vesicles using the stopped-flow/light-scattering method enabled us to evaluate the water permeability of polyprenyl phosphate vesicles with or without 10 mol% of free polyprenyl alcohol. The addition of many polyprenyl alcohols to polyprenyl phosphate vesicles decreased the water permeability, and some reduced it even more efficiently than cholesterol.

https://doi.org/10.1016/j.chembiol.2006.11.017