Wydział Chemii

Maria Jerzykiewicz

Irmina Ćwieląg-Piasecka

Maciej Witwicki

Adam Jezierski

2010

Current Topics in Biophysics

33

95-98

Naukowa

Angielski

Artykuł

Bioglycerol -1,2,3-propanotriol consisting fraction from biodiesel production-was examined towards its antioxidant activity. All investigated glycerol fractions (GF) exhibited high, in comparison with biodiesel and oil (origin material), free radical scavenging properties. Free radical scavenging activity measured by EPR spectroscopy paralleled standard antioxidant/anticorrosive investigations (Herbert Test, Folin-Ciocalteu method). Spin trapping electron paramagnetic resonance (ST EPR) spectroscopy and density functional theory (DFT) were used to study the structure of antioxidant species present in GF. The reaction with H 2 O 2 was examined via EPR measurements of formed PBN spin adducts. Additionally, pure glycerol, α-and δ-tocopherol along with oil were used as reference chemical systems, since they are potential constituents of GF. The hyperfine parameters calculated from simulated glycerol fraction-PBN adduct EPR spectra indicate the α – tocopherol origin of PBN trapped radicals. Results obtained from DFT calculations of EPR parameters stay in good accordance with experimental ones especially when the solvent effects are included in the calculations. DFT results exclude trapping of oxygen-centered radical and suggest carbon-centered type.