Repozytorium
Wyszukaj
Kolekcje
Inne
Nonenzymatic modification of ubiquitin under high-pressure and-temperature treatment : mass spectrometric studies.
Autorzy
Rok wydania
2015
Czasopismo
Journal of Agricultural and Food Chemistry
Numer woluminu
63
Strony
614-619
DOI
10.1021/jf505628e
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
The effect of high-pressure and/or high-temperature on the glycation of a model protein (ubiquitin) was investigated by mass spectrometry. This paper reports the impact of high pressure (up to 1200 MPa) on the modification of a ubiquitin using ESI-MS measurements. The application of glucose labeled with stable isotope allows a quantitative assessment of modification under the conditions of high-pressure (HPG) and high-temperature (HTG) glycation. A higher degree of modification was observed for the sample heated at 80 °C for 25 min under atmospheric pressure than for sample treated under high pressure. In samples treated at pressure below 400 MPa an insignificant increase of glycation level was observed, whereas high pressure (>600 MPa) has only a minor effect on the number of hexose moieties (Fru) attached to the lysine residue side chain.
Słowa kluczowe
glycation, high-pressure glycation, Amadori products
Adres publiczny
http://dx.doi.org/10.1021/jf505628e
Strona internetowa wydawcy
Podobne publikacje
Testing isotopic labeling with [13C6]glucose as a method of advanced glycation sites identification.
Kielmas Martyna, Kijewska Monika, Stefanowicz Piotr, Szewczuk Zbigniew
Comparison of modification sites in glycated crystallin in vitro and in vivo.
Kielmas Martyna, Kijewska Monika, Kluczyk Alicja, Oficjalska Jolanta, Gołębiewska Bożena, Stefanowicz Piotr, Szewczuk Zbigniew
The influence of glycation on a high pressure denaturation of ubiquitin.
Kijewska Monika, Radziszewska Karolina , Cal Marta, Waliczek Mateusz, Stefanowicz Piotr, Szewczuk Zbigniew