Repozytorium
Wyszukaj
Kolekcje
Inne
Nanoscale Ruthenium(III) Complexes with Bioactive Ligands: Structural, Colloidal, and Dual Antimicrobial–Cytotoxic Investigations
Autorzy
Rok wydania
2025
Czasopismo
Numer woluminu
54
Strony
14304-14321
DOI
10.1039/d5dt01857a
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
This study comprehensively analyses two new ruthenium(III) complexes, [RuIIICl4(Nic)2]−[(CH3)2NH2]+DMF, 1, and [RuIIICl2(3-HPA)2]−[3-HH2PA]+(EtOH)2, 2, (where Nic = nicotinic acid (vitamin B3), 3-HPA = anion of a 3-hydroxypicolinic acid), as potential antimicrobial agents, highlighting their physicochemical properties, nanoparticle formation, and cytotoxic activity. The complexes were fully characterised by a single crystal X-ray diffraction technique, Fourier-transform infrared, energy-dispersive X-ray, and electron paramagnetic resonance spectroscopies. The synthesis of micro- and nanoparticles (NPs) of these complexes was performed using the liquid anti-solvent crystallisation method. The formation of NPs was confirmed, and their sizes were determined using scanning electron microscopy and dynamic light scattering techniques. The Debye-Scherrer technique, based on powder diffraction X-ray data, indicated the high crystallinity of the nanomaterials. Toxicity and morphological effects on L929 fibroblasts, hepatocellular carcinoma (Hep-G2) and human epithelial colorectal adenocarcinoma (Caco-2) cell lines of the complexes were assessed using the MTT assay and an inverted phase-contrast microscope, respectively. Complex 1 is a promising anti-cancer drug candidate targeting intestinal cancers, showing cytotoxicity against Caco-2 cancer cells and no cytotoxicity against L929 fibroblast cells, while complex 2 is markedly cytotoxic. The antibacterial activity of the complexes was assessed against methicillin-resistant Staphylococcus aureus (MRSA) and Klebsiella pneumoniae strains using the minimum inhibitory concentration (MIC) method. Complex 2 demonstrates superior bactericidal properties, achieving MIC values as low as 125 μg ml−1 for S. aureus, while complex 1 exhibits lower antimicrobial efficacy. The role of ligand composition in modulating bioactivity was examined.
Licencja otwartego dostępu
Licencja na prawach której można swobodnie kopiować, rozprowadzać, zmieniać i remiksować objęty prawem autorskim utwór (Utwór-przedmiot prawa autorskiego) pod warunkiem podania imienia i nazwiska autora utworu pierwotnego oraz źródła pochodzenia utworu.
Pełny tekst licencji: https://creativecommons.org/licenses/by/3.0/pl/legalcode
Adres publiczny
http://dx.doi.org/10.1039/D5DT01857A
Strona internetowa wydawcy
Podobne publikacje
Synthesis, characterization and antitumor properties of two highly cytotoxic ruthenium(III) complexes with bulky triazolopyrimidine ligands.
Łakomska Iwona, Fandzloch Marzena, Muzioł Tadeusz, Lis Tadeusz, Jezierska Julia