ZB-3 Chemistry and Stereochemistry
         of Peptides and Proteins

 

Head of the Group: Prof. Zbigniew Szewczuk

M.Sc. 1978, Ph.D. 1982, D.Sc. 1994, Prof. 2001

 

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Ø   Visiting scientist and visiting professor at the University of Wisconsin, Medison,
USA;NRCC, Montreal, Kanada.

Ø   Member of the European Peptide Society and American Peptide Society.

Ø   Member of the Editorial Board of the Bioscience Reports.

 

Research staff:

Ø   Prof. Konopińska Danuta (emeritus)

Ø Prof. Siemion Ignacy (emeritus)

Ø Dr. Stefanowicz Piotr, D. Sc.

Ø Dr. Bartosz-Bechowski Hubert

Ø Dr. Biernat Monika

Ø   Dr. Cebrat Marek

Ø Dr. Chmieleńska Krystyna

Ø   Dr. Kluczyk Alicja

Ø Dr. Kuczer Mariola

Ø Dr. Lisowski Marek

Ø Dr. Picur Boles³aw

Ø Dr. Rusek Grzegorz

Ø B¹chor Remigiusz, M. Sc.

Ø Cal Marta, M. Sc.

Ø   Cydzik Marzena, M. Sc.

Ø   Kijewska Katarzyna, M. Sc.

Ø   Kowalewska Karolina, M.Sc.

Ø Pasikowski Pawe³, M.Sc.

Ø   Rudowska Magdalena, M.Sc.

 

 

Research topice:

 

Ø   Design and synthesis of the biologically active peptides and of their organic derivatives
(particularly as immunomodulators)

Ø   Development of new methods of the peptidomimetics and peptide conjugates synthesis

 

 

Special equipment:

 

Ø   CD measurement equipment. CD JASCO 600 with temperature control accessory.

Ø   Spectrofluorimeter.

Ø   HPLC: analytical and preparative. (Varian and Beckman System Gold, Knauer)

Ø   Capillary electrophoresis equipment. (Prince)

Ø   Polarimeter (JASCO)

Ø   Amino acid analyzer with amperometric detector (Dionex)

 

 

Recent research achievements:

 

Discovery of immunomodulatory activity of ubiquitin fragments containing retro-RGD sequence .

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Development of a new method of solid-phase synthesis of quinoxaline-containing peptides [10].

 

 

 

 

 

 

Selected papers:

 

1.       Biernat M., Stefanowicz P., Zimecki M., Szewczuk Z.
Amino-terminal dimerization of peptides on the solid support. Synthesis and biological activity of the immunosuppressive HLA-DR fragments linked by polyethylene glycols.
Bioconjugate Chem., 2006, 17, 1116-1124.

2.       Stefanowicz P., Kapczyńska K., Kluczyk A., Szewczuk Z.
A new procedure for the synthesis of peptide-derived Amadori products on a solid support.
Tetrahedron Lett., 2007, 48, 967-969.

3.       Koprowska-Ratajska M., Kluczyk A., Stefanowicz P., Bartosz-Bechowski H, Szewczuk Z.
Solid phase synthesis of peptides containing novel amino acids, substituted 3-benzimidazolealanines.

Amino Acids., 2009, 36,309-315.

4.       Jaremko £., Jaremko M., Pasikowski P., Cebrat M., Stefanowicz P., Lisowski M., Artym J., Zimecki M., Zhukov J., Szewczuk Z.
The immunosuppressive activity and solution structures of ubiquitin fragments.

Biopolymers, 2009, 91, 423-431.

5.       Kijewska M., Kluczyk A., Stefanowicz P., Szewczuk Z.

Electrospray ionization mass spectrometric analysis of complexes between peptide-derived Amadori products and borate ions.

Rapid. Commun. Mass Spectrom.,  2009, 23, 4038-4046.

6.       Stefanowicz P., Kijewska M., Szewczuk Z.

Sequencing of the peptide-derived Amadori products by the electron capture dissociation method.

J. Mass. Spectrometry, 2009, 44, 1047-1052.

7.       Stefanowicz P. Petry-Podgórska I. Kowalewska K. Jaremko £. Jaremko M. Szewczuk Z.

Electrospray mass spectrometry as a method for studying the high pressure denaturation of proteins.

Biosci. Rep., 2010, 30, 91-99.

8.       Kluczyk A., Rudowska M., Stefanowicz P., Szewczuk Z.

Microwave-assisted TFA cleavage of peptides from Merrifield resin.

J. Pept. Sci., 2010, 16, 31-39.

9.       Stefanowicz P., Kijewska M., Kapczyńska K., Szewczuk Z.

Methods of the site-selective solid phase synthesis of peptide-derived Amadori products.

Amino Acids, 2010, 38, 881-889.

10.   Stefanowicz P., Kijewska M., Kluczyk A., Szewczuk Z.

Detection of glycation sites in proteins by high resolution mass spectrometry combined with isotopic labeling.

Anal. Biochem., 2010, 400, 237-243.